Immunoblotting conditions for human hemoglobin chains
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چکیده
منابع مشابه
Heterotropic interactions in Monomeric (IS” Chains from Human Hemoglobin
The O2 affinity of fiSH chains is lowered by H+, inositol hexaphosphate (IHP), and COz. As the oxygen affinity of gSH monomers (flISH) is lower than that of fiSH tetramers (@dSH), it is possible that IHP and CO2 exert their influence on the Oz affinity of PSH chains by increasing the dissociation constant of +3dsH rather than by a direct effect on the molecule. In order to test for this hypothe...
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* This res/arch was supported by a grant from The Commonwealth Fund, Grant No. H2042(C3) from the National Heart Institute, aada grant from the National Science Foundation. 1 Fisher, H. F., E. E. Conn, B. Vennesland, and F. H. Westheimer, J. Biol. Chem., 202, 687 (1953). 2 Hoewus, F. A., F. H. Westheimer, and B. Vennesland, J. Am. Chem. Soc., 75, 5018 (1953). ' Singer, T. P., and E. B. Kearney,...
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ژورنال
عنوان ژورنال: Analytical Biochemistry
سال: 2008
ISSN: 0003-2697
DOI: 10.1016/j.ab.2008.04.008